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Sultan, S.M.; Hassan, Y. A. M.; Ibrahim, K. E. E. Sequential injection technique for automated titration: Spectrophotometric assay of vitamin C in pharmaceutical products using cerium(IV) in sulfuric acid. Analyst 124(6):917-921 (1999).

Abstract:

Sequential injection analysis (SIA) technique has been employed for titrimetry. A new SI titrimetric spectrophotometric method for the assay of vitamin C in drug formulations was explored. The method is based on the oxidation reaction of vitamin C with cerium(IV) in sulfuric acid media using a specrophotometer as a detector. A factorial design chemometric approach was employed to study the interaction effect of the chemical and system variables, mainly cerium(IV), sulfuric acid concentrations and the flow rate. Optimum operating conditions were established by chemometric optimization. The method was applied to the determination of vitamin C in pharmaceutical preparations and no excipient was found to pose any interference, thus rendering the method suitable for the determination of the drug, in pharmaceutical preparations.